Is that because of nutrient deprivation. I can not see how the difference in the 25 percent change and a fifty percent change will effect the good bacteria if the temperature, pH, salinity and dissolved oxygen levels are nearly the same. I am an engineer with a minor in chemistry though (I did organic chem, inorganic chem and analytical chem not biology), and not a biologist. What am I not thinking about or seeing that makes a difference here? My schooling in regards to bacteria is in waste water treatment, and aquatic water chemistry (for environmental engineers) which mainly deals with effects of discharges in to waterways and there effects on aquatic systems. I know many people who do 50 percent changes regularly. I do not, I use a pump and replace 4 percent daily. I understand that higher level invertebrates, as well as fish, like small or no changes to water parameters more than large changes, but I am at a loss here in regard to the larger water changes effects on the bacteria. I assume it must have something to do with something besides, pH, temp, dissolved oxygen, and nutrients. Three 25 percent water changes will probaly be about half as effective as one 50 percent water change in its effects in changing concentrations of calcium, alkalinity and magnesium. What am I missing?