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New Frontiers This is the forum to discuss new ideas and advanced topics in reefkeeping.

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Old 12-15-2003, 11:06 PM   #151 (permalink)
mojoreef
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Hmm... For the process "not" to be working, we sure have a lot of fairly successful tanks running. Couldn't it just be that ammonia is present but it is being broken down at a rate the same as it is being produced? If there were some fault in the process, ammonia would quickly overrun and leach from the substrate wouldn't it?
Not sure RC, some might just keep cycling, some get bound in organisms, some may leach in one form of nitrogen or another.

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For what it's worth, what if we're making this all more complex than it really needs to be. Maybe with a sufficient amount of surface, be it sand, plenum, mud, etc. and flow, nature is able to adapt to best make a solution for itself. There are a whole bunch of different setups who people all claim is the best, eh? (DSBs... DSBs...)
I have never personally been a big one on the just dump it in and hope all goes well, seen hundreds of tanks die that way. sand systems are complicated and I think folks need to know how they work in order to keep a healthy system.
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So this inedible "goo"... This must be pretty far down the food chain because there are some bugs who will eat pretty much anything. I still find it hard to worry about the goo with too much urgency. Some sinks are lasting a good long time.
yep they average about 3 to 4 years... so lots of time

Mike
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Old 12-15-2003, 11:22 PM   #152 (permalink)
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ummm...I'm feelin kinda dumb here right now...so how do we get arround this particular obstacle?
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Old 12-15-2003, 11:26 PM   #153 (permalink)
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Sorry Scott "goo" was just an easier word then to list all the components of it
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Ok, so the ammonium (NH4) is not broken down initially broken down into N2 but instead, incoorporated into amino acids and perhaps ultimatly, proteins (which might also be a good sink for our sulfur since disulfide bonds are important to protein tertiary structure). This protein can either act as a sink or be broken down by some sort of scavenger, thus giving the nitrogen the oppurtunity to be gassed off again?
Yep the ammonium could be have the chance to off gas, but only if thier was no presence of ammonia. The ammonia would not allow for the first enzyne in the dinitrifiaction process to occur. I think it does a little of all of what you are saying. Most protiens are that of the bacteria that they use for thier reducing (by products). One would assume that it would be used to some degree by critter though as energy sources.
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I have never seen in the literature that a protein goo acts as a sink. Is this just speculation or is this actually a pretty consistent dogma in the hobby?
Ok lets not use the word goo anymore, my bad, lol. Look at it this way, a collection of enzynes, bacterial flock, particulate dust, end product detritus, food/waste, microbes and so on that are all used or are biproducts of biochemical cycles. or just goo if you want, hehe. This is not really hobby stuff but more in the marine biology stuff.

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It seems that there either has to be this goo, N2 is gassed off, or there is some other sink because most of our tanks have very low nitrogen readings (if properly maintained)...
agreed . do you think the sink is locked in the substraight??? if it coould be locked or sunk in the substraight or plenum area I guess it could still be viable with good maintence. If it leaches that would make it a scrub. I think I will email charles back and see if he monitored any leaching.

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Old 12-15-2003, 11:29 PM   #154 (permalink)
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Maxx for everything that is not nitrogen based a sand substriaght is a sink, so....maybe as long as this doesnt leach, maybe it being a nitrogen sink can also work. If we sink all this stuff in the plenum, maybe that is a good thing, then at least we know where it is, lol.

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Old 12-15-2003, 11:58 PM   #155 (permalink)
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I'm going to digest this for awhile....I've had to tap into the lost resources of my brain, clean out the cobwebs, and now am trying to direct my thoughts in a constructive way....why do I feel dense all of the sudden? lol
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Old 12-16-2003, 12:15 AM   #156 (permalink)
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Originally posted by mojoreef
yep they average about 3 to 4 years... so lots of time

Mike
Cough, Cough (16), cough...

-Rogue :P
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Old 12-16-2003, 10:24 AM   #157 (permalink)
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agreed . do you think the sink is locked in the substraight??? if it coould be locked or sunk in the substraight or plenum area I guess it could still be viable with good maintence. If it leaches that would make it a scrub. I think I will email charles back and see if he monitored any leaching.
It makes sense to me that this "goo" or in more scientific terms, this proteinaceous accumulation would be a great sink for our nitrogenous wastes as well as other contiminants. It is something I have never thought of but makes perfect sense. As for it contributing to a sudden crash, I am unsure. It seems that these accumulations of amino acids would have the nitrogen bound pretty tightly although bacteria would always be breaking it down.

I am starting to get a bit confussed about this protein because it appears that it came from bacteria and will be continually processed by bacteria. If so, then is it like a nutrient storage type idea or are different species of bacteria working at this stuff? If it is cyclical, then eventually the nitrogen would have to be gassed off at some point or as I mentioned, there has to be some other sink involved (because the nitrogen in our tank is zero). I wonder what the nitrogen (NH3, NO3, etc...) readings would be in the plenum space...

Ah, I almost need to chart all of this out as a biochemical pathway to get it straight in my head. I love this stuff but man it is so hard to keep every idea straight in your head...

Oh, and definitly let me know what you hear from Charles and also, there is no better word in the world to describe a huge mass of non-differentiated amino acids as "goo," be it in the scientific community or otherwise.

I will be back for more.

Take er easy
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Old 12-16-2003, 10:35 AM   #158 (permalink)
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Originally posted by RogueCorps
Cough, Cough (16), cough...

-Rogue :P
Okay so Rob Toonan had a tank that lasted 16 years w/o crashing. He later broke down and parted out things for reasons unrelated to OTS or a crash of somesort. But how many others do you hear about like that? I don't think that if you looked at it in straight percentages that you'd get very many going over the 6 year mark. It would be an interesting study at least. Maybe not the most useful, but certainly interesting.
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Old 12-16-2003, 11:35 AM   #159 (permalink)
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Maxx Robs tank had 2 fish and some soft corals and was run by a marine biologist, I dont know if one could say that is the average system. Reef central ran a poll on DSB's (not a real scientific poll but a poll all the same) from it of all the folks with DSB's thier were ony 24 that actually lasted over 4 years. 51 failed after 2 years, 40 that failed between 2-4 years and 5 that failed after 5 years. But really alot to do about nothng. The concept here on this forum and this board is not really to argue what is best but is to know how they work. with this knowledge it is easy to find the merits of any system.

Scott I am sorry if I am being a little confusing in my discriptions, I am not very good at relaying what I know.
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It makes sense to me that this "goo" or in more scientific terms, this proteinaceous accumulation would be a great sink for our nitrogenous wastes as well as other contiminants. It is something I have never thought of but makes perfect sense. As for it contributing to a sudden crash, I am unsure. It seems that these accumulations of amino acids would have the nitrogen bound pretty tightly although bacteria would always be breaking it down.
I agree, the protienaceous waste also has alot of other things mixed in it, but for the sake of this problm lets stick to just this. I think your post hits it pretty much on the head.
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I am starting to get a bit confussed about this protein because it appears that it came from bacteria and will be continually processed by bacteria. If so, then is it like a nutrient storage type idea or are different species of bacteria working at this stuff?
More like the nitrogen bouncing between inorganic and organic. Look at it this way, the nitrogen based products will always be off gassed unles thier is a presence of ammoina. If thier is a presence it cant o down the road of off gassin because the protien needed canto not be made. If this case occurs the nitrogen based stuff goes down a different road and basically turns to organic N and the cycle starts over.
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If it is cyclical, then eventually the nitrogen would have to be gassed off at some point or as I mentioned, there has to be some other sink involved (because the nitrogen in our tank is zero). I wonder what the nitrogen (NH3, NO3, etc...) readings would be in the plenum space...
Yep I was seeing a little light on this setup until I got Charlies reply saying that the ammonia concintration in the plenum and subsraight was 3 times as much as in natural water. This is kinda how this latest side track started.

Thanks for the discussion, keep it coming.

Mike
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Old 12-16-2003, 12:23 PM   #160 (permalink)
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In this system, is there tubes running down like an UGF?
I was studying a friends FW, UGF system, I help set-up. At one end, is air pumped down the tube, flowing water through the filter. On the other side I installed a PH that sucks water down the tube & from the UGF. He mentioned how he doesn't really need to clean his filter floss anymore. I'm sure by now, being months set-up it has tons of waste underneath. If I had a drain in the bottom center & drained as mentioned, would this in effect be doing the same as what we have here? Would there be benefit to add an array like an UGF and suck water through it?
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Old 12-16-2003, 07:36 PM   #161 (permalink)
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Scott itwas one of the ideas. the tube would be thier but would not be in operation. They would be used only a few times a year. The plenum set up with screening would allow water and so on to be sucked through the substraight. was that what you were asking???

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Old 12-16-2003, 10:37 PM   #162 (permalink)
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Quote:
Originally posted by ScottT1980

Ah, I almost need to chart all of this out as a biochemical pathway to get it straight in my head. I love this stuff but man it is so hard to keep every idea straight in your head...

Scott, I'm with you here...I need a pathway at this point to understand everything that we are talking about.

One thing I came across in my biochemistry text....some believe that nitrate reductase is repressed by ammonia. Any thoughts on this?
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Old 12-16-2003, 10:52 PM   #163 (permalink)
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While I am a bit strapped for time, I will see what I can do with all of this in the next couple of days and try to get some sort or "editable" schematic going that we can add to so that we all stay ont he same page. No guarentees but I will do my best...

Take er easy
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Old 12-16-2003, 11:39 PM   #164 (permalink)
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Scott, you're awesome if you could do that. I think you have a pretty good handle on this, and I really think it will be helpful for me to see the biochemical pathways visually, instead of picturing them in my brain, make sense?
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Old 12-16-2003, 11:43 PM   #165 (permalink)
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I was following along until recently...now I'm desperately needing a schematic to keep up...I'm good w/ bio but thisoutside my area of knowledge.
Nick
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