Ity Bity

PootyTang · 11-05-2009, 06:59 PM

So I know this is kind of a weird question, and a very scientific one to be asking on a reef site, but does anyone know of any stains that are good for observing diatoms under a microscope? I've got the scope at home and I'll order the stain on eBay, but I have no idea which to use.
I thought it would be cool to get a pic through the microscope, probably a good way to win POTM don't u think?

BLAKEJOHN · 11-05-2009, 07:01 PM

Give heinze a pm. He would know this one for sure.

PootyTang · 11-05-2009, 07:32 PM

Sorry, what's his username? i tried heinze and it didn't find it

BigAl07 · 11-05-2009, 09:26 PM

Heinze = hma

Rhodes19 · 11-05-2009, 10:09 PM

It it was bacteria I would say use a gram + or -. Maybe iodine. Can your microscope do dark field?

PootyTang · 11-05-2009, 11:14 PM

no, it can't do dark field (although that would be pretty cool), I'm beginning to think that might be the only decent way to see them though, since they're eukaryotic and have a silica shell, i don't know any stains that would penetrate that. I PMed heinze though, so we'll see

Rhodes19 · 11-06-2009, 08:57 AM

Quote:

Originally Posted by PootyTang

no, it can't do dark field (although that would be pretty cool), I'm beginning to think that might be the only decent way to see them though, since they're eukaryotic and have a silica shell, i don't know any stains that would penetrate that. I PMed heinze though, so we'll see

Let us know what he has to say. You definitly have my interest. Might have to break out my scopes.

11-05-2009, 06:59 PM	#1 (permalink)
PootyTang Pistol Shrimp Join Date: Mar 2009 Posts: 253 View Gallery Karma Komment	Ity Bity So I know this is kind of a weird question, and a very scientific one to be asking on a reef site, but does anyone know of any stains that are good for observing diatoms under a microscope? I've got the scope at home and I'll order the stain on eBay, but I have no idea which to use. I thought it would be cool to get a pic through the microscope, probably a good way to win POTM don't u think?

11-05-2009, 07:01 PM	#2 (permalink)
BLAKEJOHN Elegance coral Join Date: Mar 2008 Location: KINGSTON, I.L. Posts: 1,073 View Gallery Karma Komment	Re: Ity Bity Give heinze a pm. He would know this one for sure. __________________ 110 gallon acrylic 1 Yellow Tang, 1 Blue Tang, 2 Eng Gobies, 1 Six Line Wrasse, 1 Tomato Clown, 1 Lion fish RBTA, Longspine Urchin, Approx. 200 snails Red Monti, Purple Rim Monti, Leathers, Elegence, Yellow Polyps, various Zoa's, FrogSpawn, Ricordias, Greenbirdsnest, Pink Milli, Blue Milli, Bonzai, unknown Acropora's, Purple Digi, GSP, Bubble Coral, Staghorn, Slimer

11-05-2009, 07:32 PM	#3 (permalink)
PootyTang Pistol Shrimp Join Date: Mar 2009 Posts: 253 View Gallery Karma Komment	Re: Ity Bity Sorry, what's his username? i tried heinze and it didn't find it

11-05-2009, 09:26 PM	#4 (permalink)
BigAl07 Do I look as lost as I am Join Date: Mar 2007 Location: Waynesville, North Carolina Posts: 21,549 View Gallery Karma Komment Add yours!	Re: Ity Bity Heinze = hma __________________ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ~~~~~~~~~~~~ *Live Rock Rubble will do the SAME thing as Bio-Balls and is NOT a suitable replacement for BIO-BALLS in a Reef System! It's ALL gotta go!!* Nitrate (NO3) reduction is directly proportional to percentage of Water Change. Allen's home-made formula...currentNO3-((%WC.01)currentNO3)=finalNO3 (thanks Luukosian) This means if you change 50% of your total water volume (That's EVERYTHING) you'll get a net reduction of (NO3) somewhere around 50%. Ask me about how to increase your REEF budget without going without FOOD!!* Big Al's 10g Julie's (BigAl's Gal) 6g NanoCube Gone but not forgotten BigAl's Slow 90g Tank Chronicle Allens OFFICE 12g Nano-Reef

11-05-2009, 10:09 PM	#5 (permalink)
Rhodes19 Scopas Tang Join Date: Jul 2009 Location: Charleston, SC Posts: 402 View Gallery Karma Komment	Re: Ity Bity It it was bacteria I would say use a gram + or -. Maybe iodine. Can your microscope do dark field? __________________ 180 reef in the build 29 reef Biocube 14 reef 45 SWFO 45 FW 10 reef My wife notices any additions to the tanks. "Is that new"? "And if my wife asks, it was only $20!" (bodyguard295) My Addiction Rhodes19's 180 build

11-05-2009, 11:14 PM	#6 (permalink)
PootyTang Pistol Shrimp Join Date: Mar 2009 Posts: 253 View Gallery Karma Komment	Re: Ity Bity no, it can't do dark field (although that would be pretty cool), I'm beginning to think that might be the only decent way to see them though, since they're eukaryotic and have a silica shell, i don't know any stains that would penetrate that. I PMed heinze though, so we'll see

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